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23. A nucleic acid vector for use in in vivo shipping of the nucleic acid of interest into a qualified receiver bacterial cell, stated nucleic acid of fascination manufacturing a presented impact on claimed focused receiver bacterial mobile, whereby said vector comprises: said nucleic acid of desire, and
wherein, once sent into stated specific receiver bacterial mobile, said nucleic acid of curiosity makes explained presented impact on reported targeted receiver bacterial mobile although said vector isn't replicated in mentioned qualified receiver bacterial mobile.
whereby, the moment sent into mentioned qualified receiver bacterial cell, mentioned 官方網 nucleic acid of desire provides said supplied effect on explained qualified receiver bacterial cell although mentioned vector just isn't replicated in reported targeted receiver bacterial mobile.
By “host endogenous molecule” is meant herein any molecule Normally made by the host subject matter, especially by a healthful host topic.
due to the fact phages Have a very exact tropism to the identical or closely connected species in which These are developed, the packaged phagemids derived from this phage, at the time their payloads shipped while in the focus on germs, will keep replicating, Except the phage has been engineered to infect/inject in a whole new group of microorganisms.
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In a particular embodiment, antibiotic resistant strains are targetly killed by programming the nuclease to complete a DNA cleavage, e.
In a specific embodiment, when claimed vector can be a packaged phagemid, explained vector does not comprise any element derived from your organism from which the conditional origin of replication is derived.
Unless of course defined normally, all technical and scientific terms made use of herein contain the same indicating as usually understood by one among standard talent inside the art to which this creation belongs.
As applied herein, “acknowledged by” with respect to the restriction web site and a restriction enzyme signifies that the restriction web page is cleaved by the restriction enzyme.
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Third, they analyzed in vitro killing of E. coli and as opposed it to the current era of replicative payloads.
a hundred and forty、根据本公开的感兴趣的核酸可包含编码cas蛋白的核酸序列。多种crispr酶可用于用作质粒上感兴趣的序列。在一些实施方案中，crispr酶为ii型crispr酶。在一些实施方案中，crispr酶催化dna切割。在一些其他实施方案中，crispr酶催化rna切割。优选地，crispr酶不造成双链断裂。在一些实施方案中，crispr酶造成单链断裂或切口。在一些实施方案中，crispr酶不会在dna或rna中造成任何断裂。在一个实施方案中，cas13-脱氨酶融合被用于对rna进行碱基编辑。
The inventors clearly show for The 1st time that phagemids is usually packaged at high titers using a conditional ORI with ori and protein needed for replication in trans,